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| Rodríguez,Edisleidy; Betancourt,A; Acevedo,Ana María; Relova,Damarys; Ayala,J; Barrera,Maritza. |
| La gastroenteritis transmisible (TGE) es una enfermedad de los cerdos, infecciosa, aguda y altamente contagiosa; causada por un virus de la familia Coronaviridae, género Coronavirus. En nuestro país, a partir del año 2003 se comenzaron a presentar brotes de síndrome diarreico con las características clínico epizotiológicas de la enfermedad de TGE, pero para lograr la confirmación del diagnóstico era necesario aislar el virus en cultivos celulares a partir de animales enfermos, seguido de una identificación en aquellos cultivos que presentaran efecto citopático, para lo cual era necesario desarrollar una metodología para el aislamiento viral, ya que para este virus ha sido reportado con anterioridad que el aislamiento en células resulta muy difícil de... |
| Tipo: Journal article |
Palavras-chave: Gastroenteritis transmisible del cerdo; TGE; Aislamiento viral. |
| Ano: 2008 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200004 |
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| Rodríguez,Edisleidy; Betancourt,A; Ancizar,J.A; Joa,R; López,A; Relova,Damarys; Barrera,Maritza. |
| The experimental reproduction of transmissible gastroenteritis was achieved in newborn piglets, after inoculation by oral route of a strain of this virus isolated in Cuba. In both cases, diarrheas appeared at 24 hours post-inoculation. In all inoculated piglest, anatomo and histopathological lesions which corresponded to the disease were observed in necropsy. Transmissible gastroenteritis virus was excreted after the establishment of diarrhea and not any other pathogenic microorganism involved was found. This sustained the enteropathogenicity of the isolate. |
| Tipo: Journal article |
Palavras-chave: Transmissible gastroenteritis of pigs; Experimental reproduction; Enteropathogenicity. |
| Ano: 2008 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000300005 |
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| Acevedo,Ana María; Rodríguez,Edisleidy; Uffo,Odalys; Relova,Damarys; Noda,Julia; Díaz de Arce,Heidy. |
| Avian leukosis viruses (ALV) provoke a variety of trasmissible bening and malign tumoral diseases affecting birds. Chickens are affected by six subgroups of ALV designs A, B, C, D, E and J of more recent world dissemination. These viruses are potential contaminants of live vaccines used in poultry. In order to research the presence of DNA from ALVs as contaminants of viral commercial vaccines to be used in poultry, different Marek´s disease vaccines were screened by a reported polymerase chain reaction (PCR) assay designed to detect all subgroups of ALVs. DNA samples extracted from seven vaccines were submitted to PCR using primers for a conserved region of env gene of HPRS-103. ALV sequences were detected in seven samples (100%). The methodology employed... |
| Tipo: Journal article |
Palavras-chave: Avian leukosis virus (ALV); Polymerase chain reaction (PCR); Vaccine; Contamination; Poultry. |
| Ano: 2009 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000100011 |
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